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il 32a  (R&D Systems)


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    Structured Review

    R&D Systems il 32a
    Il 32a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+32a/Recombinant+Human+IL-32+alpha+Protein%2C+CF/pm35143819-110-30-35
    Average 92 stars, based on 19 article reviews
    il 32a - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Incubation:

    Article Title: Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1.
    Article Snippet: .. Membranes were incubated with antibodies against IL-32 (513501, BioLegend; or MAB30401, R&D Systems), IL-32a (AF3040, R&D Systems), and tubulin (1:1000; TS168, Sigma-Aldrich) overnight 10 of 13 SC I ENCE ADVANCES | R E S EARCH ART I C L E D ow nloaded from https://w w w .science.org on Septem ber 06, 2024 at 4°C. .. After washing the membranes three times for 10 min, they were incubated with horseradish peroxidase–conjugated anti-mouse (A9044, Sigma-Aldrich), anti-rat (A 9037, Sigma-Aldrich), or anti-goat (V8051, Promega) antibodies for at least 1 hour.

    Cell Culture:

    Article Title: IL-32 Supports the Survival of Malignant T Cells in Cutaneous T-cell Lymphoma.
    Article Snippet: For cultures of T cells alone, T cells were further purified using negative magnetic bead selection on the AutoMACS Pro (Miltenyi Biotec, Bergisch Gladbach, Germany) with the Pan T cell Isolation Kit (catalog number 130-096-535, Miltenyi Biotec). .. A total of 300,000e1,000,000 cells in 200 ml per well were cultured in round-bottom 96-well plates in the presence or absence of IL-2 (25 IU/ml, National Cancer Institute, Bethesda, MD), IL-32a (25 ng/ml; number 3040-IL-050, R&D Systems), IL-32b (25 ng/ml; number 6769-IL-025, R&D Systems), or IL-32g (25 ng/ml; number 4690-IL-025/CF, R&D Systems). ..



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